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J Korean Acad Pediatr Dent. 2010;37(3): 275-287.
GENE EXPRESSION ANALYSIS OF THE DENTAL PULP IN HEALTHY AND CARIES TEETH
So-Hee Oh1, Jong-Soo Kim2
1Hallym Sacred Heart Hospital
2Department of Pediatric Dentistry, School of Dentistry, Dankook University
Corresponding Author: Jong-Soo Kim ,Tel: 041-550-1935, Email: jskim@dku.edu
Received: April 2, 2010;  Accepted: August 2, 2010.
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ABSTRACT
Deep caries may induce pulpitis and the pulpal tissue interacts with microbial invasion. The immune response to protect the pulpal tissue can be mediated by cellular signal molecules produced by the pulpal cells. The understanding of these processes is important to find future therapeutic method for the diseased pulp. The pulp tissue from sound teeth was set as control group (n=30) and the pulp tissue from decayed teeth was set as test group (n=30). Total RNA was extracted from the pulp of each group and it was used for cDNA microarray and reverse transcriptase-polymerase chain reaction(RT-PCR). The expression of TGF-β1 was studied by immunohistochemistry. The results were as follows: 1. cDNA microarray analysis identified 520 genes with 6-fold or greater difference in expression level with 143 genes more abundant in health and 377 genes more abundant in disease. 2. The RT-PCR analysis was done for randomly selected 14 genes and the results supported the result of cDNA microarray assay. 3. TGF-β1 was highly expressed in the carious pulp and it was found in odontoblast by immunohistochemistry. In conclusion, many cytokines were found to be significantly changed their expression in the diseased pulp(/M/>1.6).
Keywords: Caries | cDNA microarray analysis | RT-PCR analysis | Immunohistochemistry
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