Journal of the Korean Academy of Pediatric Dentistry 2010;37(3):275-287.
Published online August 31, 2010.
GENE EXPRESSION ANALYSIS OF THE DENTAL PULP IN HEALTHY AND CARIES TEETH
So-Hee Oh1, Jong-Soo Kim2
1Hallym Sacred Heart Hospital
2Department of Pediatric Dentistry, School of Dentistry, Dankook University 
치아 우식증에 따른 치수내 유전자 발현 변화에 관한 분석
오소희1, 김종수2
1한림대학교 성심병원 소아치과
2단국대학교 치과병원 소아치과
Correspondence:  Jong-Soo Kim,  Tel: 041-550-1935, Email: jskim@dku.edu
Received: 2 April 2010   • Accepted: 2 August 2010
Abstract
Deep caries may induce pulpitis and the pulpal tissue interacts with microbial invasion. The immune response to protect the pulpal tissue can be mediated by cellular signal molecules produced by the pulpal cells. The understanding of these processes is important to find future therapeutic method for the diseased pulp. The pulp tissue from sound teeth was set as control group (n=30) and the pulp tissue from decayed teeth was set as test group (n=30). Total RNA was extracted from the pulp of each group and it was used for cDNA microarray and reverse transcriptase-polymerase chain reaction(RT-PCR). The expression of TGF-β1 was studied by immunohistochemistry. The results were as follows: 1. cDNA microarray analysis identified 520 genes with 6-fold or greater difference in expression level with 143 genes more abundant in health and 377 genes more abundant in disease. 2. The RT-PCR analysis was done for randomly selected 14 genes and the results supported the result of cDNA microarray assay. 3. TGF-β1 was highly expressed in the carious pulp and it was found in odontoblast by immunohistochemistry. In conclusion, many cytokines were found to be significantly changed their expression in the diseased pulp(/M/>1.6).
Key Words: Caries, cDNA microarray analysis, RT-PCR analysis, Immunohistochemistry


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