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J Korean Acad Pediatr Dent. 2004;31(2): 304-313.
THE EFFECT OF XYLITOL ON THE EXPRESSION OF GTF GENE
염정현1, 정진2, 정태성1, 김신1
1부산대학교 치과대학 소아치과학교실
2부산대학교 치과대학 구강미생물학교실
Corresponding Author: 김신 ,
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ABSTRACT
Xylitol, a five-carbon natural sugar alcohol, is widely used non-cariogenic sugar substitute. In present study, the effects of xylitol on the expression of mRNA for glucosyltransferase which synthesizes glucan from sucrose were detected by Fluorescent in situ hybridization (FISH) and flow cytometry. FITC fluorescences for mRNA of gtfB, gtfC and gtfD were decreased further with increasing concentration of xylitol from 1% to 10% when detected by FISH. Flow cytometric analysis also showed that the expression of gtfB, gtfC and gtfD was increased by the addition of sucrose and decreased by the addition of xylitol to BHI broth containing 1% sucrose. In conclusion, the expression of gtfB, gtfC and gtfD mRNA was decreased by the addition of xylitol.
Keywords: Xylitol | Streptococcus mutans | gtf gene | mRNA | FISH | Flow cytometry
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