| THE EFFECTS OF IONS AND BUFFER SOLUTIONS ON THE MRNA EXPRESSION OF gtfD GENE OF Streptococcus mutans |
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| Streptococcus mutans의 gtfD 유전자 발현에 대한 이온 및 완충액의 영향 |
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김보영1, 김신2, 정진1 |
1부산대학교 치과대학 미생물학교실 및 구강생물공학 연구소
2부산대학교 치과대학 소아치과학교실 |
Correspondence:
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| Abstract |
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The production of a glucan was affected by the concentration of ions and buffer solutions, and nutrients in an oral cavity. In this study, the effects of ions and buffer solutions on the mRNA expression of gtfD gene in Streptococcus mutans, an important causative agent of dental caries, were investigated by Fluorescent in situ hybridization(FISH). At first, ions and buffer solutions had little effect on the multiplication of Streptococcus mutans. The green fluorescence according to the mRNA expression of gtfD gene was detected in the BHI broth containing 1% sucrose. The intensities of the green fluorescence were strong at 0.25mM of CaCl2. Little fluorescence was detected by the addition of KCl, except far 10mM KCl at which fluorescence intensities were similar to those of the control. Fluorescence intensities were weak at each concentration of MgCl2 when compared to the control. As for buffer solutions, fluorescence intensities were similar to those of the control at each concentration of buffer solutions, except that they were little detected at 100mM of potassium phosphate. |
| Key Words:
Streptococcus mutans, Glucan, Ion, Buffer |
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