Journal of the Korean Academy of Pediatric Dentistry 2013;40(3):185-193.
Published online August 31, 2013.
Effects of Mineral Trioxide Aggregate on the Proliferation and Differentiation of Human Dental Pulp Stromal Cells from Permanent and Deciduous Teeth
Seunghye Kim1, Mijeong Jeon1, Dong Min Shin2, Jae Ho Lee1, Je Seon Song1
1Department of Pediatric Dentistry, College of Dentistry and Oral Science Research Center, Yonsei University, Seoul, Korea
2Department of Oral Science, College of Dentistry, Yonsei University, Seoul, Korea 
Mineral trioxide aggregate가 유치 및 영구치의 치수기질세포 증식 및 분화에 미치는 영향
김승혜1, 전미정1, 신동민2, 이제호1, 송제선1
1연세대학교 치과대학 소아치과학교실, 구강과학연구소
2연세대학교 치과대학 구강생물학교실
Correspondence:  Je Seon Song,  Tel: +82-2-2228-3176, Fax: +82-2-392-7420, Email: SONGJS@yuhs.ac
Received: 30 November 2013   • Accepted: 4 June 2013
Abstract
Mineral trioxide aggregate (MTA) has recently been used as a pulpotomy medicament for primary molars. The aim of this study was to evaluate and compare the proliferation and differentiation potential of dental pulp stromal cells of permanent teeth and deciduous teeth cultured on MTA-coated surface. Human dental pulp stromal cells were obtained from human permanent premolars and deciduous teeth and cultured on MTA-coated culture plates. The cells were subjected to proliferation assay and cell cycle analysis. Their differentiation potential was evaluated by analysing changes in the mRNA expressions of runt-related transcriptional factor 2 (Runx2) and alkaline phosphatase (ALP). Morphological changes of cells in direct contact with MTA were observed using scanning electron microscopy (SEM). The proliferation rates, distribution of cell cycles and mRNA expression patterns of Runx2 and ALP were similar in both types of pulpal cells. SEM observations revealed that both types changed into more dendrite-like cells. On the surface of MTA, human dental pulp stromal cells from deciduous and permanent teeth were able to both proliferate and differentiate into cells that induce mineralization. MTA is suitable as a biocompatible pulpotomy medicament for primary teeth.
Key Words: Mineral Trioxide Aggregate (MTA), Human dental pulp stromal cells, Deciduous teeth, Permanent teeth, Proliferation, Differentiation


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