PERIODONTOPATHIC BACTERIA AND ANTIBIOTIC RESISTANCE GENES OF ORAL BIOFILMS IN CHILDREN

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Journal of the Korean Academy of Pediatric Dentistry 2011;38(2):170-178.
Published online May 31, 2011.

PERIODONTOPATHIC BACTERIA AND ANTIBIOTIC RESISTANCE GENES OF ORAL BIOFILMS IN CHILDREN

¹전남대학교 치의학전문대학원 소아치과학교실 및 치의학연구소
²전남대학교 치의학전문대학원 치의학교육학교실 및 치주학교실
³전남대학교 치의학전문대학원 교정학교실
⁴전남대학교 치의학전문대학원 치의학교육학교실
⁵전남대학교 의과대학 미생물학교실

어린이 치면세균막에서 치주질환원인균과 항생제 내성유전자의 출현율

김선미¹, 최남기¹, 조성훈¹, 이석우², 임회정³, 임회순⁴, 강미선⁵, 오종석⁵

Correspondence: ,

Received: 13 April 2011 • Accepted: 10 May 2011

Abstract

The purpose of this study was to assess the prevalence of periodontopathic bacteria and resistance determinants from oral biofilm of children. Subgingival dental plaque was isolated from 87 healthy children, and PCR was performed to determine the presence of 5 periodontal pathogens including P. gingivalis, T. forsythia, T. denticola, F. nucleatum, A. actinomycetemcomitans, and nine resistance genes including tet(Q), tet(M), ermF, aacA-aphD, cfxA, bla_SHV, bla_TEM, vanA, mecA. 1. The prevalence of F. nucleatum, T. forsythia. and P. gingivalis was 95.4%, 55.2%, and 40.2%, respectively. In addition. the prevalence of A. actinomycetemc omitans was 5.7%, while T. denticola was 3.4%. 2. In analysis of antibiotic resistance determinants. cfxA, bla_TEM and tet(M) were detected in all the samples tested. It was also found that the prevalence of tet(Q) showing tetracycline resistance. bla_SHV associated with resistance to β-lactams, ermF exhibiting erythromycin resistance, and, vanA resulting vancomycin resistance was 88.5%, 29.9% 87.4%, and 48.5%, respectively. The aacA-aphD gene showing resistance to aminoglycosides and mecA gene harboring methicillin resistance exhibited the lowest prevalence with 9.2%. 3. In a correlation analysis between periodontopathic pathogens and antibiotic resistance determinants, it was found that there was a significant correlation between T. forsythia and bla_SHV. Also, P. gingivalis and vanA showed a correlation. Finally, tet(Q) and ermF showed a significant correlation (phi: 0.514) while mecA and vanA also showed a correlation(phi: 0.25).

Key Words: Periodontopathic bacteria, Antibiotic resistance, Antibiotic resistance determinants, Oral biofilm, Polymerase chain reaction