THE CYTOTOXICITY OF PULPOTOMY MEDICAMENTS ON HUMAN PULP FIBROBLAST CELLS |
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치수 절단술 약제의 치수 섬유모세포에 대한 세포독성 연구 |
이영희, 이긍호 |
경희대학교 치과대학 소아치과학교실 |
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Abstract |
The effect of concentration as factor in cytotoxicity, protein synthesis and alkaline phosphatase activity was compared for pulpotomy medicaments (formaldehyde, formocresol, paraformaldehyde, ferric sulfate) Human pulp fibroblasts were exposed to a range of concentrations(0.01, 0.05, 0.10, 0.25, 0.50, 1.00μl/ml) of each agents, for period of 24hrs. The cell activities were evaluated by MTT assay, protein assay and alkaline phosphatase activity examination. The results as follows : 1. After 24hrs culture, pulp fibroblasts adding formaldehyde, formocresol and paraformaldehyde were suppressed cell activities with concentration increasing, but, no depression of cell activities by ferric sulfate. No significant difference was in formaldehyde, formocresol and paraformaldehyde. 2. Protein synthesis by pulpotomy agents were not significant difference in pulp fibroblasts but protein synthesis were a little decreased by paraformaldehyde. 3. Alkaline phosphatase activity was a little decreased by pulpotomy medicaments. |
Key Words:
Pulpotomy medicaments, Cytotoxicity, Pulp fibroblasts |
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