| Localization of Dendroaspis natriuretic peptide (DNP) in the rat salivary glands |
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| 흰쥐 타액선내에서의 DENDROASPIS NATRIURETIC PEPTIDE(DNP)의 분포 |
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김재곤, 이영수, 백병주, 박병건 |
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전북대학교 치과대학 소아치과학교실 |
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| Abstract |
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Dendroaspis natriuretic peptide (DNP), a fourth member of the natriuretic peptide isolated from the venom of the Dendroaspis angusticeps snake, has been reported to be present in human plasma and atrial myocardium and caused vasorelaxation and diuresis in experimental animals. However, it is uncertain whether they are present in peripheral organs other than the heart and its further physiological roles also remains to be clarified. To assess the possible physiological role of DNP in the salivary glands, I investigated the localization of DNP peptide in the rat salivary glands by immunohistochemistry and the binding sites for radiolabelled DNP in the rat salivary glands and oral mucosa using in vitro autoradiography. DNP immunoreactivity was widely distributed in the submandibular, sublingual and parotid glands, particularly in the ducts such as the intercalated and striated ducts, where atrial natriuretic peptide (ANP) was colocalized in consecutive sections, but not in acini. High density 125I-DNP binding sites were localized in the epithelia of the tongue and hard palate, while low density binding sites for 125I-DNP were also distributed in the submandibular, sublingual, and parotid glands. In the hard palate and tongue, the precise location of this binding was revealed on the basal and parabasal cells of the epithelia by emulsion microautoradiography. These results suggest that DNP may not only have a role in the salivary glands but also play a role in the regulation of growth in the oral epithelium, particularly in the hard palate and tongue. |
| Key Words:
Dendroaspis natriuretic peptide(DNP), Salivary glands, Immuno histo-chemistry |
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