| A COMPARATIVE STUDY OF PRESERVING ABILITY OF HUMAN PERIODONTAL LIGAMENT CELLS STORED IN DIFFERENT STORAGE MEDIA |
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| 수종의 저장용액에서 치주인대세포의 생존율 비교 |
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최원경, 최형준, 최병재, 이종갑 |
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연세대학교 치과대학 소아치과학교실 |
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| Abstract |
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Preservation of the remaining periodontal ligament cells on an avulsed tooth is very important to the successful outcome of replantation. HBSS is recommended as the most suitable storage medium for the avulsed tooth that cannot be replanted immediately. But their availability near the site of an accident is doubtful. The purpose of this in vitro study was to compare periodontal ligament cells stored in different storage media obtained easily on the spot. Human periodontal ligament cells were collected from the premolar teeth extracted for orthodontic treatment. Cells were cultured in α-MEM culture medium containing 20% FBS, at 37℃ 100% humidity, in a 5% CO2 incubator. Cells were cultured in 96 well culture plate, 5×104 cells per well with α-MEM and incubated for 24 hours. After discarding the medium, those cells were cultured in α-MEM contained with 10% FBS, pasteurized milk, sterilized saline, unstimulated saliva and bench-dried state at 25℃ room temperature for 30, 60, 90, 120, 180 minutes respectively. And then each group was measured using MTT assay. The results were as follows. 1. Between the group of each time, there was statistically significant difference. Periodontal ligament cells viability was highest in pasteurized milk and was reduced stepwisely in sterilized saline, unstimulated saliva and bench-dried state(p<0.05). 2. between the time of each group, there was statistically significant difference(p<0.05) but was no statistically significant difference at 90-120 minutes in pasteurized milk and at 60-90 minutes and 120-180 minutes in sterilized saline(p>0.05). In conclusion, HBSS as storage medium of an avulsed tooth is not practical on the spot. Insteadily pasteurized milk can be recommended to maintain the periodontal ligament cells viability. |
| Key Words:
MTT assay, periodontal ligament cell viability, storage media |
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