| THE ASPECT OF PROLIFERATION AND BONE NODULE FORMATION IN OSTEOBLAST-LIKE CELLS DERIVED FROM FETAL RAT CALVARIA IN VITRO |
| |
| 백서 태자 두 개관에서 유래된 조골세포의 증식 및 골결절 형성양상 |
|
김시형, 남순현, 신홍인 |
|
경북대학교 치과대학 소아치과학 교실 |
|
|
|
|
| Abstract |
|
The purpose of this study was to investigate the aspects of proliferation and bone nodule formation of osteogenic precursor cells. To determine the effects of ascorbic acid and dexamethasone upon capacity of osteoblast proliferation and bone nodule formation, cells were maintained in the presence of one or some of these additives for up to 30 days. Group I culture was maintained in standard medium(DMEM plus 10% plus antibiotics), group II was maintained in supplemented medium containing dexamethasone, group III was maintained in supplemented medium containing ascorbic acid and sodium-β-glycerophosphate, and group IV was maintained in supplemented containing ascorbic acid, sodium-β-glycerophosphate and dexamethasone. Morphology of bone nodules was observed with light microscope and electron microscope. The results were as follows: ● Proliferation capacity of osteoblasts was not affected by single use of dexamethasone, but it was chiefly affected by ascorbic acid. ● Cellular morphology was fibroblastic appearance initially, but, it was gradually changed to polygonal shape accompanied by confluency stage. ● Pluripotent mesenchymal cells existed during primary culture, they were differentiated to adipocyte, chondrocyte, osteocyte according to culture condition. ● Dexamethasone increased bone nodule formation under the condition that the culture was maintained with supplemented medium ascorbic acid and sodium-β-glycerophosphate. ● when the cultures were stained with alizarin red, the group supplemented with dexamethasone, ascorbic acid and sodium-β-glycerophosphate showed the marked increase of bone nodule formation, but the group supplemented with ascorbic acid and sodium-β-glycerophosphate revealed only small amounts of bone nodules. And the groups cultured without ascorbic acid showed no observed any of bone-like mass independent of dexamethasone addition. |
|
PDF Links
Download Citation
