A STUDY ABOUT THE INDUCTION OF MUTANASE FROM STREPTOMYCES

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Journal of the Korean Academy of Pediatric Dentistry 1996;23(3):764-773.
Published online August 31, 1996.

A STUDY ABOUT THE INDUCTION OF MUTANASE FROM STREPTOMYCES

¹
²

Streptomyces의 mutanase 유도에 관한 연구

양규호¹, 정진²

¹전남대학교 치과대학 소아치과학교실
²전남대학교 치과대학 미생물학교실

Abstract

The mutan containing α-1,3 bond is an insoluble portion of glucan which is the main component of dental plaque. The secretion of mutanase was assessed with mutan-digesting Streptomyces isolated from soil, and the factors affecting its activity was studied, obtaining the following result. Mutan-digesting Streptomyces was identified as Streptomyces exfoliatus by its characteristics. The effect of dextranase was identified on the media containing blue dextran. A clear zone was produced by Streptomyces exfoliatus on the media containing blue mutan, so showing the secretion of mutanase. A clear zone was significantly produced on the media overlayed with agar containing blue mutan. A clear zone was produced at 2 days after the inoculation of Streptomyces exfoliatus on the media containing below a concentration of 0.025% glucose, at 3 days on the media containing 0.05 % glucose, and at 4 days on the media containing 0.1 % glucose. Mutan-digestion wasn't appeared early by adding other carbohydrates. The higher concentration of peptone, the later appearance of clear zone was on the media containing below a concentration of 0.1 % peptone. These results indicated that the secretion of mutanase was identified from mutan-digesting Streptomyces on the media containing blue mutan, and a clear zone was appeared lately on the media containing higher amount of glucose.