Journal of the Korean Academy of Pediatric Dentistry 2003;30(4):728-737.
Published online November 30, 2003.
CARIES-RELATED MICROBIOLOGICAL SCREENING IN CHILDREN UNDER THREE YEARS OF AGE
Jae-Hong Park, Keung-Ho Lee
 
3세이하의 어린이의 치아우식증과 관련된 미생물학적 선별검사
박재홍, 이긍호
경희대학교 치과대학 소아치과학교실
Correspondence:  Jae-Hong Park, 
Abstract
To evaluate microbial data and salivary measurements from clinically compatible, culture-based screening procedures employed with children younger than 36 months old. Plaque and stimulated saliva specimens were collected from 87 children. The pH of each saliva sample was measured before and after 0.94% lactic acid was added. Specimens were diluted and plated on selective media and non-selective media. Data collected were counts of mutans streptococci (MS) and lactobacilli (LB). In addition, total viable counts (TVC) of specimens, salivary pH and buffering capacity were also assessed. Each variable was compared to caries status of subjects. According to this study, the results were as followed: 1. Highly significant correlation with caries rates were found for counts of MS and LB. 2. The specific counts/ml saliva or plaque above which caries is predicted, or below which caries is not predicted were as follows: 1) Saliva MS; 105 2) Plaque MS; 2×105 3) Saliva LB; 103 4) Plaque LB; 103. 3. Salivary pH and buffering capacity versus caries status were not significant. 4. Microbial screening methods based on mutans streptococci had higher predictive values and odds ratios than methods for lactobacilli. 5. MS counts were clearly the best indicators of caries status in young children. This measurement can easily be obtained in a dental clinical setting both by conventional culture techniques, or commercial kits for MS recovery.
Key Words: Plaque, Stimulated saliva, Mutans streptococci, Lactobacilli, Salivary pH, Buffering capacity
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