| INHIBITION OF BIOFILM FORMATION BY PEDIOCOCCUS PENTOSACEUS K1270 ISOLATED FROM KIMCHI |
|
Woi-Im Choi1, Su-Ji Han2, Shin Kim1 |
1
2 |
| Pediococcus pentosaceus K1270에 의한 인공치태 형성억제 효과 |
|
최외임1, 한수지2, 김신1 |
1부산대학교 치과대학 소아치과학교실
2피츠버그대학 약학대학 약학과 약물유전학센터 |
Correspondence:
Shin Kim,
|
|
|
|
|
| Abstract |
|
Pediococcus pentosaceus K1270 was isolated from naturally fermented kimchi and identified based on the 16S rDNA sequence as well as cultural and biochemical characteristics. This strain strongly inhibited the formation of biofilm by Streptococcus mutans Ingbritt. K1270 also showed antibacterial activity against S. mutans Ingbritt. It was observed that K1270 strain produced hydrogen peroxide on MRS agar supplemented with 3, 3, 5, 5-tetramethylbenzidine (TMB) and peroxidase and the inhibitory effect of K1270 strain on the biofilm formation was reversed by the addition of catalase in part. Culture supernatant of K1270 inhibited the biofilm formation and the multiplication of S. mutans Ingbritt. This inhibitory effect of culture supernatant was decreased slightly by the addition of catalase and abolished by heat or trypsin treatment. Thus, this study suggests that P. pentosaceus K1270 inhibit the biofilm formation through the inhibition of the replication of S. mutans Ingbritt by producing hydrogen peroxide and bacteriocin. |
| Key Words:
Biofilm, Kimchi, Inhibition of dental plaque, S. mutans, P. pentosaceus |
|
PDF Links
Download Citation
