Journal of the Korean Academy of Pediatric Dentistry 2009;36(1):12-19.
Published online February 28, 2009.
THE ANTIBIOTIC ACTIVITY OF ACTINOMYCES ISOLATED FROM PLAQUE OF BLACK STAINED PRIMARY TEETH TO STREPTOCOCCUS MUTANS
Soo Jin Park, Shin Kim, Tae Sung Jeong, Jae Moon Kim
Department of Pediatric Dentistry, School of Dentistry, Pusan National University 
Black stain을 가진 유치 치태에서 추출한 방선균의 S. mutans에 대한 항생능 평가
박수진, 김신, 정태성, 김재문
부산대학교 치의학전문대학원 소아치과학교실
Correspondence:  Soo Jin Park,  Tel: 051-240-7449, Email: liebelang@hanmail.net
Received: 27 August 2008   • Accepted: 17 November 2008
Abstract
The aim of this study is to assess the antibiotic activity of Actinomyces in plaque from black stained primary teeth to Streptococcus mutans. Samples were obtained from four children, 2-6 years of age, who had black stains on all erupted primary teeth. 16 different Actinomyces spp. were isolated, and antibiotic activity test with paper disc method was done. The results were as follows, 1. No.1 and No.5 Actinomyces spp. showed the antibiotic activity to Streptococcus mutans and the activity of No.5 Actinomyces spp. could compete with that of Oxacillin. 2. No.1 and No.5 Actinomyces spp. also exhibited the antibiotic activity to Bacillus cereus, Bacillus subtilis commonly used as experimental bacteria for testing antibiotic activity. 3. For identification of No.1 and No.5 spp., PCR analysis was done. No.5 spp. matched Actinomyces viscosus at 97% level but No.1 spp. didn't match.
Key Words: Black stain, Actinomyces, Antibiotic activity, S. mutans.


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