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J Korean Acad Pediatr Dent. 2008;35(1): 73-82.
Cloning and expression of Streptococcus mutans GS-5 glucosyltransferase
Su-Kyeong Kim, Jae-Gon Kim, Byeong-Ju Baik, Yeon-Mi Yang, Kyung-Yeol Lee, Jeong-Yeol Park
Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University
Corresponding Author: Jae-Gon Kim ,Tel: 063-250-2121, 2223, Fax: 063-250-2131, Email: pedodent@chonbuk.ac.kr
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ABSTRACT
Dental caries is an infectious disease caused by mutans streptococci, and is a primary etiologic agent of dental caries in humans. The molecular pathogenesis of mutans streptococcal-associated dental caries occurs in three phases. Firstly, S. mutans attaches to tooth surface via a cell surface adhesion termed antigen I/II. In the second phase, the glucosyltransferase(GTFs) synthesize polymers like glucans in the presence of sucrose. In the third phase, the multivalent glucans interacts with glucan binding proteins (GBPs) and they make dental plaque and accumulation of microorganisms. Many studies and clinical trials have indicated that a mucosal immune response to these antigens(Ag I/II, GTFs, GBPs) of S. mutans can influence the pathogenesis of dental caries. So these antigens can be important vaccine candidates for immunologic intervention against dental caries. In this study, we cloned the genes for GTFb, GTFc, GTFd from S. mutans GS-5 and did the nucleotide sequence analysis. And the recombinant proteins of GTFd and N-terminus of GTFd were expressed. Intact GTF which we get from this experiment can be used for antibody production specific for any GTF activity domain through animal experiment.
Keywords: Streptococcus mutans | Glucosyltransferase | Glucan
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