THE ANALYSIS OF REMINERALIZATION EFFECT IN FLUORIDE VARNISH USING CONFOCAL LASER SCANNING MICROSCOPE

Kwon, Ji-Hoon; Park, Ho-Won; Lee, Ju-Hyun; Seo, Hyun-Woo

Journal of the Korean Academy of Pediatric Dentistry 2008;35(1):57-64.
Published online February 29, 2008.

THE ANALYSIS OF REMINERALIZATION EFFECT IN FLUORIDE VARNISH USING CONFOCAL LASER SCANNING MICROSCOPE

Ji-Hoon Kwon, Ho-Won Park, Ju-Hyun Lee, Hyun-Woo Seo

Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Kangnung National University

Correspondence: Ju-Hyun Lee, Tel: 033-640-3158, Email: ljh55@kangnung.ac.kr

Abstract

It is well established that fluoride products play an important role in the prevention and remineralization of carious lesion. Fluoride varnish is a concentrated topical fluoride and varnishes adhere to tooth surface, permitting prolonged fluoride exposure and uptake. In this study, the artificial initial enamel caries was caused on the sound human enamel and divided 60 specimens into three groups. Group 1 and group 2 were treated with the topical application of fluoride varnish and stored in artificial saliva for 1 and 2 weeks. Group 3 was stored in artificial saliva for 2 weeks, which acted as control group. Changes in mineral contents were analysed with the confocal laser scanning microscope. The following results were obtained: 1. In group 1 and group 2, the total fluorescence of the lesion(TFL) was reduced in remineralized area compared to in demineralized area(p<0.05). 2. The total fluorescence of the lesion of remineralized area was more reduced in group 2 than in group 1(p<0.05). 3. The total fluorescence of the lesion was more reduced in group 2 than in control group(p<0.05). 4. Confocal laser scanning microscope can be used in quantitative analysis of remineralization by fluoride varnish.

Key Words: Fluoride varnish, Remineralization, Confocal laser scanning microscope