| THE EFFECT OF RISPERIDONE ON SALIVARY GLAND CELLS |
|
Yeon-Joo Lee, Yeong-Jae Kim, Jung-Wook Kim, Ki-Taek Jang, Chong-Chul Kim, Se-Hyun Hahn, Sang-Hoon Lee |
| Department of Pediatric Dentistry, College of Dentistry and Dental Research Institute, Seoul National University |
| 리스페리돈이 타액선 세포에 미치는 영향 |
|
이연주, 김영재, 김정욱, 장기택, 김종철, 한세현, 이상훈 |
|
서울대학교 치과대학 소아치과학교실 및 치학연구소 |
Correspondence:
Sang-Hoon Lee, Tel: 02-2072-2680, Email: musso@snu.ac.kr
|
|
|
|
|
| Abstract |
|
Risperidone is a widely prescribed atypical antipsychotic agent. Approved by the FDA as the first drug to treat irritability associated with autism in children, it is also used to treat tic disorder and Tourette’s syndrome. Its adverse reactions related to dentistry include dry mouth, the mechanism of which is yet to be identified. The aim of this study is to identify, at the cellular level, how and to what extent risperidone affects intracellular free calcium concentration ([Ca2+]i), an primary intracellular factor in the regulation of fluid secretion in salivary gland cells. The human salivary gland cell line (HSG) was grown in MEM supplemented with 10% BCS. In order to measure [Ca2+]i, Fura-2/AM was loaded in the HSG, and fluorescence at 340 nm/380 nm excitation was measured in the 500 nm emission ratio. After every experiment, a calibration experiment was conducted in order to readjust the ratio to the actual [Ca2+]i. Changes in [Ca2+]i were measured in the presence of carbachol, ATP and histamine. The r searcher then explored how the pretreatment of risperidone affected such changes Findings of this study include: 1. In HSG, [Ca2+]i increased due to the addition of carbachol, ATP and histamine. The presence of risperidone inhibited the action of histamine on this process, while making little effect on that of carbachol and ATP. 2. A quantification of [Ca2+]i in relation to histamine of different concentrations indicates that the effect of histamine was concentration dependent with an EC50 of 3.3±0.5 μM. 3. The inhibitory effect of risperidone on histamine-induced [Ca2+]i was concentration-dependent with an IC50 of 104.4±14 nM. 4. Risperidone inhibits histamine-induced Ca2+ release from endoplasmic reticulum and influx of extracellular Ca2+ in HSG cells(p<0.05). |
| Key Words:
Risperidone, Human salivary gland cells, Intracellular calcium concentration, Histamine, Carbachol, ATP |
|
PDF Links
Download Citation
