| Quantitative comparison of mRNA expression of glucosyltransferase (GTF) between xylitol-resistant(XR) and xylitol-sensitive(XS) mutans streptococci |
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MI-Na Lee1, Young-Jae Kim1, Sung-Hoon Lee2, Chong-Chul Kim1 |
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| Mutans streptococci의 자일리톨 내성균주와 감성균주의 glucosyltransferase mRNA의 정량적 비교 연구 |
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이미나1, 김영재1, 이성훈2, 김종철1 |
1서울대학교 치의학대학원 소아치과학교실 및 임상치의학연구소
2서울대학교 치의학대학원 구강악안면 감염 및 면역학교실 |
Correspondence:
Chong-Chul Kim,
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| Abstract |
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Since the long-term exposure of mutans streptococci to xylitol is known to select for xylitol-resistant (XR) natural mutants, the occurrence and survival of such (XR) strains were performed in batch culture methods. The aim of the study was to compare the differentiation and quantification of mRNA expression of the gtf genes of XRand XS mutans streptococci. Using a real-time reverse-transcription polymerase chain reaction, the expression of each gtf was determined. In XR strains, the relative levels of transcription of gtfB and gtfC were decreased while that of gtfD was increased, suggesting the presence of independent promoters. It also suggested that mutation related to production of glucosyltransferase occurred under the exposure of xylitol could explain the caries-preventive mechanisms of xylitol. |
| Key Words:
Xylitol resistant(XR), gtf, Glucosyltransferase |
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