THE ANTIMICROBIAL EFFECT OF HORSERADISH(ARMORACIA RUSTICANA) ROOT EXTRACTS AGAINST STREPTOCOCCUS MUTANS ISOLATED FROM HUMAN DENTAL PLAQUE |
Hye-Kyoung Kim1, Ho-Won Park1, Il-Shik Shin2, Ju-Hyun Lee1, Hyun-Woo Seo1 |
1Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Kangnung National University 2Faculty of Marine Bioscience and Technology, Kangnung National University |
치태에서 분리된 Streptococcus mutans에 대한 서양산 고추냉이(Armoracia rusticana) 뿌리 추출물의 항균효과 |
김혜경1, 박호원1, 신일식2, 이주현1, 서현우1 |
1강릉대학교 치과대학 소아치과학교실 및 구강과학연구소 2강릉대학교 해양생명공학부 식품미생물학연구실 |
Correspondence:
Ho-Won Park, Tel: 033-640-3157, Email: pedo@kangnung.ac.kr |
|
|
Abstract |
Recently interesting in development of antimicrobial agent from natural origin has been increased in these days. Many studies have been reported antimicrobial effect of Horseradish(Armoracia rusticana) root extracts against various microorganisms such as Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Vibrio parahaemolyticu, Staphylococcus aureus, Bacillus subtilis, Aspergillus parasiticus, Helicobacter pylori. The main component related to antimicrobial activity in horseradish is well known as allyl isothiocyanate(AIT). In this study, we investigated the antimicrobial effects of Horseradish(Armoracia rusticana) root extracts against Streptococcus mutans isolated from human dental plaque, Streptococcus mutans reference strain and compared with that of chlorhexidine. Horseradish root extracts and chlorhexidine were tested to determine their minimum inhibitory concentration( MIC) and minimum bactericidal concentration(MBC). The result of this study can be summerized as follows; 1. Horseradish root extracts showed antimicrobial effect against both S. mutans isolated strain and reference strain, their MIC were respectively 0.083~0.25% (833.33~2500 ppm), 0.25% (2500 ppm). Horseradish root extracts showed antimicrobial effect against S. mutans isolated strain at same or slightly lower concentration compared with MIC of reference strain. 2. 0.083~0.25% horseradish root extracts showed similar antimicrobial effect with chlorhexidine (0.0021~0.0041%). |
Key Words:
Horseradish(Armoracia rusticana) root extracts, Allyl isothiocyanate(AIT), Streptococcus
mutans, Chlorhexidine, 16s rRNA sequence, API kit |
|