Journal of the Korean Academy of Pediatric Dentistry 2009;36(3):358-366.
Published online August 31, 2009.
THE EFFECT OF FERMENTED MILK ON VIABLE CELL COUNT AND BIOFILM FORMATION OF STREPTOCOCCUS MUTANS
Hye-Sung Shin1, Seon-Mi Kim1, Nam-Ki Choi1, Kyu-Ho Yang1, Mi-Sun Kang2
1Department of pediatric dentistry, Chonnam national university school of dentistry, Dental Research Institute and Second stage of BK21
2Department of Microbiology, Collge of Medicine 
유산균 발효유가 Streptococcus mutans의 생균수 및 biofilm 형성에 미치는 영향
신혜성1, 김선미1, 최남기1, 양규호1, 강미선2
1전남대학교 치의학전문대학원 소아치과학교실, 치의학 연구소 및 2단계 BK 21,
2의과대학 미생물학교실
Correspondence:  Seon-Mi Kim,  Tel: 062-530-5668, Email: hellopedo@hanmail.net
Received: 18 March 2009   • Accepted: 30 June 2009
Abstract
actic acid bacteria worked positively on gastrointestinal tract and oral environment. So I selected commercial five fermented milks and milk, and then I evaluated their effect of growth inhibition and biofilm formation of cariogenic bacteria, Streptococcus mutans. And also calculated the acidity, buffering capacity, concentration of Ca and P ion and pH change of those drinks. After adding S. mutans to fermented milks viable cell count of S. mutans in milk was not statistically different but those in all fermented milks were decreased as concentration of fermented milk increased. When I measured the amount of formed biofilm in 10% fermented milks and milk with S. mutans and compared with those without S. mutans, the amount was decreased in Active GG and Bulgaris while being increased in Tootee, Ace and milk(P<0.05). The fermented milk with the lowest pH value was E5(3.48±0.01), and the highest was Bulgaris(4.19±0.02). pH change of the fermented milks and milk with S. mutans was measured. The highest acid producing fermented milk was Bulgaris, and followed by Active GG, Ace, Tootee, E5, Milk. These results indicated that fermented milks had caries activity due to the value of initial acidity and acid producing capacity. But, concentrated fermented milks had the inhibitory effect against S. mutans, and also had high volume of Ca and P ion that protected teeth. So I suggest that they have positive effect on teeth.
Key Words: S. mutans, Fermented milk, Biofilm


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