| EFFECT OF THE NUCLEAR FACTOR I-C ON THE FORMATION OF HERTWIG'S EPITHELIAL ROOT SHEATH DURING ROOT DEVELOPMENT |
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In-Cheol Shin1, Joo-Cheol Park1, Moon-Jin Jeong1, Hyun-Ju Oh1, Sun-Hwa Park2, Chang-Seop Lee3, Heung-Joong Kim2 |
1Department of Oral Histology & BK21, Chosun University
2Oral anatomy, College of Dentistry, Chosun University
3Pediatric Dentistry, College of Dentistry, Chosun University |
| Nuclear factor I-C가 치근발생 과정에서 Hertwig's 상피초 형성에 미치는 영향 |
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신인철1, 박주철1, 정문진1, 오현주1, 박선화2, 이창섭3, 김흥중2 |
1조선대학교 치과대학 구강조직학교실 및 BK21
2조선대학교 치과대학 구강해부학교실
3조선대학교 치과대학 소아치과학교실 |
Correspondence:
Heung-Joong Kim, Tel: 062-230-6875, Email: hjbkim@chosun.ac.kr
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| Abstract |
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Tooth formation is a complex developmental process that is mediated through a series of reciprocal epithelial-mesenchymal interactions. Several signal pathways and transcription factors have been implicated in regulating molar crown development, but relatively little is known about the regulation of root development. It was reported that NFI-C knockout mice showed abnormal root formation with normal crown. The aims of this study are to elucidate how the NFI-C regulate the determine of root shape and odontoblasts differentiation. We carried out immunohistochemistry using cytokeratin to investigate the role of Hertwig's epithelial root sheath and DSPP mRNA in-situ hybridization to conform the nature of root dentin during root development in NFI-C knockout mice. Cytokeratin reacted with all the HERS cells and the continuity of cytokeratin positive cells between the HERS cells and enamel epithelium was lost in the cervical region both wild and K/O types. After root dentin deposition cytokeratin positive-HERS cells showed irregularity and loss of polarity in the cervical region in K/O type. DSPP mRNA was strongly expressed in odontoblasts of crown and root dentin in wild type mice, whereas expression of DSPP mRNA was restricted in odontoblast of crown dentin in the K/O type. During root formation in NFI-C knockout mice, HERS normally grow out of the crown but fail to induce odontoblast differentiation in root portion. These results suggest that NFI-C may play important roles in odontoblast differentiation during root dentin formation. |
| Key Words:
NFI-C knockout, Odontoblast differentiation, Root formation, Cytokeratin, DSPP |
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