THE INHIBITORY EFFECT OF FRUCTAN-PRODUCING S. SALIVARIUS ON THE FORMATION OF ARTIFICIAL PLAQUE |
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Fructan 생성 S. salivarius의 인공치태 억제효과 |
박소영1, 박은혜1, 오종석2, 양규호1 |
1전남대학교 치과대학 소아치과학교실 및 치의학 연구소 2전남대학교 의과대학 미생물학교실 |
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Abstract |
S. mutans is the most important causative bacteria of dental caries among the oral bacteria. S. salivarius is a normal inhabitant in the human oral cavity. Nine strains of S. salivarius in this study were isolated from the oral cavities of children and identified, and their effect on S. mutans and S. oralis was studied. 1. The mean weight of produced artificial plaque on the wires in the beaker was 204.9 mg in the culture of S. mutans only, whereas being reduced to 1.9 mg through 20.6mg in the combined culture of S. mutans and each S. salivarius isolate (p<0.05). The viable cell didn't show the difference between them after culturing. 2. When S. mutans was cultured in the media containing culture supernatant of each S. salivarius isolate in M17 broth, the mean weight of produced artificial plaque was 117.1 mg on the wires, whereas being 47.7 mg in the media containing culture supernatant of each S. salivarius isolate in M17 broth containing 5% sucrose. 3. The polymer produced by S. salivarius isolates was on the thin layer chromatography. 4. Inulin and levan didn't inhibit the formation of artificial plaque by S. mutans in the beaker test. These results suggested that fructan-producing S. salivarius isolates inhibited the formation of artificial plaque by S. mutans. |
Key Words:
S. salivairus, Artificial plaque, Fructan |
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