FACTORS AFFECTING THE PRODUCTION OF SULFUR COMPOUNDS BY FUSOBACTERIUM NUCLEATUM

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Journal of the Korean Academy of Pediatric Dentistry 2003;30(1):33-40.
Published online February 28, 2003.

FACTORS AFFECTING THE PRODUCTION OF SULFUR COMPOUNDS BY FUSOBACTERIUM NUCLEATUM

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²

Fusobacterium nucleatum의 유황화합물 생성에 영향을 미치는 인자

오인근¹, 박은혜¹, 오종석², 양규호¹

¹전남대학교 치과대학 소아치과학 교실 및 치의학 연구소
²전남대학교 의과대학 미생물학교실

Abstract

Fusobacterium nucleatum, one of the bacteria causing halitosis, produces the volatile sulfur compounds (VSC) such as H₂S in the media containing sulfur components, and forms FeS by binding with iron component. The various factors of oral cavity affect the concentration of sulfur compounds produced by Fusobacterium nucleatum. In this study, the effect of nutrients and pH on the production of sulfur compounds by Fusobacterium nucleatum was studied with the following results. 1. The optical density of broth was increased to 0.817±0.032 and 1.297±0.024 by adding 1.0% sodium thiosulfate and 0.05% L-cysteine hydrochloride in the media, respectively. 2. Though the optical density of broth was 0.799±0.032 by adding volatile sulfur compounds (VSC) only in the media, it was increased to 1.775±0.003 and 1.648±0.022 by adding xylitol combined with glucose and fructose, respectively. 3. The concentration of VSC was above 20,000 ppb in the media above pH 5.5. The optical density of broth was still high in the media with L-cysteine hydrochloride of higher concentration, being low in the media of lower pH. 4. The concentration of VSC was high when there was distilled water or saline solution on the media, and their amount was small. These results suggest that the production of sulfur compounds by Fusobacterium nucleatum was inhibited by xylitol and acid.

Key Words: Fusosacterium nucleatum, Volatile Sulfur Compounds (VSC), Xylitol