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J Korean Acad Pediatr Dent. 2010;37(2): 186-192.
DETECTION OF SALIVARY STREPTOCOCCUS MUTANS LEVELS USING MONOCLONAL ANTIBODIES
Chu-Sung Kim, Jae-Gon Kim, Yeon-Mi Yang, Byeong-Ju Baik, Kyung-Yeol Lee, Mi-Ah Kim, Su-Min Lim
Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University
Corresponding Author: Jae-Gon Kim ,Tel: 063-250-2128, Email: pedodent@chonbuk.ac.kr
Received: February 17, 2010;  Accepted: May 7, 2010.
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ABSTRACT
Streptococcus mutans, one of the major causal agents of dental caries, is component of the dental plaque. It produces various organic acids such as lactic acid which is the end-product of glycolysis, and this leads to dental caries. A new system using species-specific monoclonal antibodies was developed to detect Streptococcus mutans in saliva. The system quickly detects salivary Streptococcus mutans in 30min and classifies the result into two levels. The purpose of this study was to investigate correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods. Children’s deft indices were also compared with Streptococcus mutans counts in MSB agar plate. Subjects consisted of 15 children in the age of 3 to 6 years. They were assigned to three groups : Group Ⅰ (deft index = 3), Group Ⅱ(deft index ≤ 3), Group Ⅲ(deft index ≥ 4). The results are as follows : 1. The rate of children with positive response was 13.3% and with negative response was 86.7% in the result of Saliva-checkTM Mutans test kit. 2. There was a positive correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods(p<0.05). 3. Streptococcus mutans counts in MSB agar plate were irrelevant to deft of children(p=0.34 ).
Keywords: Dental caries | Streptococcus mutans | Monoclonal antibody
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