| DETECTION OF SALIVARY STREPTOCOCCUS MUTANS LEVELS USING MONOCLONAL ANTIBODIES |
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Chu-Sung Kim, Jae-Gon Kim, Yeon-Mi Yang, Byeong-Ju Baik, Kyung-Yeol Lee, Mi-Ah Kim, Su-Min Lim |
| Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University |
| 단클론항체를 이용한 타액 내 Streptococcus mutans 수준의 측정 |
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김추성, 김재곤, 양연미, 백병주, 이경열, 김미아, 임수민 |
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전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소 |
Correspondence:
Jae-Gon Kim, Tel: 063-250-2128, Email: pedodent@chonbuk.ac.kr
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Received: 17 February 2010 • Accepted: 7 May 2010 |
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| Abstract |
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Streptococcus mutans, one of the major causal agents of dental caries, is component of the dental plaque. It produces various organic acids such as lactic acid which is the end-product of glycolysis, and this leads to dental caries. A new system using species-specific monoclonal antibodies was developed to detect Streptococcus mutans in saliva. The system quickly detects salivary Streptococcus mutans in 30min and classifies the result into two levels. The purpose of this study was to investigate correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods. Children’s deft indices were also compared with Streptococcus mutans counts in MSB agar plate. Subjects consisted of 15 children in the age of 3 to 6 years. They were assigned to three groups : Group Ⅰ (deft index = 3), Group Ⅱ(deft index ≤ 3), Group Ⅲ(deft index ≥ 4). The results are as follows : 1. The rate of children with positive response was 13.3% and with negative response was 86.7% in the result of Saliva-checkTM Mutans test kit. 2. There was a positive correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods(p<0.05). 3. Streptococcus mutans counts in MSB agar plate were irrelevant to deft of children(p=0.34 ). |
| Key Words:
Dental caries, Streptococcus mutans, Monoclonal antibody |
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