EFFECTS OF COLLAGENASE AND ESTERASE ON DENTIN BOND STRENGTH AND NANOLEAKAGE

Jung, Young-Jung; Hahn, Se-Hyun; Kim, Chong-Chul; Lee, Sang-Hoon; Kim, Jung-Wook; Kim, Young-Jae; Jang, Ki-Taeg

Journal of the Korean Academy of Pediatric Dentistry 2008;35(3):389-398.
Published online August 31, 2008.

EFFECTS OF COLLAGENASE AND ESTERASE ON DENTIN BOND STRENGTH AND NANOLEAKAGE

Young-Jung Jung, Se-Hyun Hahn, Chong-Chul Kim, Sang-Hoon Lee, Jung-Wook Kim, Young-Jae Kim, Ki-Taeg Jang

Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University

Collagenase와 esterase가 상아질 접착강도와 nanoleakage에 미치는 영향

정영정, 한세현, 김종철, 이상훈, 김정욱, 김영재, 장기택

서울대학교 치과대학 소아치과학교실 및 치학연구소

Correspondence: Ki-Taeg Jang, Tel: 02-2072-2682, Email: jangkt@snu.ac.kr

Received: 23 April 2008 • Accepted: 6 May 2008

Abstract

The purpose of this study was to evaluate the effects of collagenase and esterase on dentin bond strength and nanoleakage. Resin composites were bonded to occlusal dentin of premolars with Single Bond 2(SB) and Clearfil SE Bond(SE). After the microtensile specimens were prepared and stored in PBS for 24 hours(I) or, PBS(II), collagenase(III), esterase(IV) solution for 4 weeks, the specimens were stained with silver nitrate solution. Microtensile bond strength(μTBS) and silver penetration area were measured and, the results were as follows: 1. For group II, III, and IV, the bond strengths of SB were lower than those of SB(p<0.05). The bond strengths of SB II, III, and IV were lower than that of SB I(p<0.05). There was no difference among the bond strengths of SE I ~ IV groups(p>0.05). 2. Silver penetration areas of SB were higher than those of SE for all storage groups(p<0.05). In SB and SE groups, there was no significant difference of silver penetration area among I ~ IV groups(p>0.05). 3. SE I, II, and III showed inverse relationship between the bond strengths and the silver penetration areas(p<0.05).

Key Words: Collagenase, Esterase, Bond strength, Nanoleakage