QUANTITATIVE DETECTION OF PERIODONTOPATHIC BACTERIA USING REAL-TIME PCR

Kim, Seon-Mi; Yang, Kyu-Ho; Choi, Nam-Ki; Kang, Mi-Sun; Oh, Jong-Suk

Journal of the Korean Academy of Pediatric Dentistry 2008;35(3):494-503.
Published online August 31, 2008.

QUANTITATIVE DETECTION OF PERIODONTOPATHIC BACTERIA USING REAL-TIME PCR

Seon-Mi Kim¹, Kyu-Ho Yang¹, Nam-Ki Choi¹, Mi-Sun Kang², Jong-Suk Oh²

¹Department of Pediatric Dentistry, School of Dentistry, Chonnam National University and Dental Research Institute and second stage of BK 21,
²Department of Microbiology, College of Medicine, Chonnam National University

Real-time PCR을 이용한 치주질환 원인균의 정량적 분석

김선미¹, 양규호¹, 최남기¹, 강미선², 오종석²

¹전남대학교 치의학전문대학원 소아치과학교실 및 치의학연구소 및 2단계 BK21
²전남대학교 의과대학 미생물학교실

Correspondence: Seon-Mi Kim, Tel: 062-220-5476, Email: smkim1406@hanmail.net

Received: 11 March 2008 • Accepted: 28 April 2008

Abstract

The detection of special bacteria of oral plaque and quantitative analysis are possible through the real-time PCR technique. The aim of this study was to investigate the prevalence and the amount of five periodontopathic bacteria using real-time PCR, and evaluate the relationship among amount of bacteria, plaque index and gingival index. Sixty five subjects ranging from 8 to 18 years old were enrolled. Plaque index and gingival index were examined and five periodontopathic bacteria were surveyed in subgingival plaque samples. The results were analyzed by ANOVA and Pearson's correlation. Results: The prevalence of P. gingivalis was 61.5%, T. forsythia was 53.8%, T. denticola was 29.2%, A. actinomycetemcomitans was 15.4% and F. nucleatum was 100%. Plaque index and gingival index have a significant relationship with the amount of F. nucleatum. The amount of T. forsythia has a strong relationship with the amount of T. denticola and A. actinomycetemcomitans(p<0.05).

Key Words: Periodontopathic bacteria, Real-time PCR, Plaque index, Gingival index