IDENTIFICATION OF THE AG I/II AND GTFD GENES FROM STREPTOCOCCUS MUTANS GS-5 |
Jin-Woo Jeong, Byeong-Ju Baik, Yeon-Mi Yang, Jeong-Ah Seo, Jae-Gon Kim |
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연쇄상구균 GS-5의 ag I/II와 gtfD 유전자 클로 |
정진우, 백병주, 양연미, 서정아, 김재곤 |
전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소 |
Correspondence:
Jae-Gon Kim, |
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Abstract |
Streptococci are Gram-positive, facultative anaerobes and have no catalase activities. Among mutans streptococci containing α-type hemolytic activity, S. mutans is a causative agent for dental caries. As well as acid production yielding the demineralization of tooth enamel, adherence and colonization of S. mutans to the teeth are also important for its virulence. These early colonization are accomplished by the bacterial fibrillar protein, Antigen I/II (Ag I/II) and glucosyltransferase (GTF). Therefore, Ag I/II and GTF are reasonable targets for the development of vaccine against S. mutans GS-5. The ag I/II and gtfD genes from S. mutans GS-5 were cloned and sequenced. Sequence analyses showed the nucleotides sequence of cloned genes had high homology to the sequences previously reported. The sequence alignment of 280 nucleotides between the cloned Ag I/II and the available sequence of the corresponding S. mutans GS-5 showed the perfect match. Comparing with the sequence of gtfD from S. mutans UA159, the corresponding nucleotide sequence of S. mutans GS-5 showed some mismatches and the mismatches introduced changes in four residues out of 105 amino acids, yielding four missense mutations. |
Key Words:
Streptococcus mutans, Antigen I/II, Glucosyltransferase |
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