| EXPRESSION OF DSPP AND BSP MRNAS DURING ODONTOBLAST DIFFERENTIATION IN THE NFI-C (-/-) MICE |
1
2
3 |
| NFI-C 결손 생쥐의 상아모세포 분화과정에서 DSPP와 BSP mRNA의 발현 |
|
정현구1, 이상호1, 이난영1, 김흥중2, 박주철3, 이창섭1 |
1조선대학교 치과대학 소아치과학교실 구강생물학연구소
2조선대학교 치과대학 구강해부학교실 구강생물학연구소
3조선대학교 치과대학 구강조직학교실 구강생물학연구소 |
Correspondence:
,
|
|
|
|
|
| Abstract |
|
Nuclear factor I (NFI) exists in the odontoblast and osteoblast. NFI-C null mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation, and molar lacking roots. The purpose of this study was to examine phenotype of the aberrant odontoblast in NFI-C null mice and to evaluate the expression of DSPP and BSP mRNAs in NFI-C null mice with in-situ hybridization. The results were as follows: 1. In the NFI-C (-/-) mice, the crown dentin of molar showed normally formation, but there was no root dentin. 2. In the NFI-C (-/-) mice, the labial dentin of mandibular incisors showed relatively a lot of dentin formation, but the lingual dentin showed defect. 3. In the NFI-C (-/-) mice, the odontoblast of mandibular incisors revealed abnormal shape and trapped in osteodentin-like mineralized tissue. 4. In the NFI-C (-/-) mice, the odontoblast in the crown dentin of molars showed strong expression of DSPP, the odontoblast in the root dentin of molars was not expression of DSPP. In the NFI-C (-/-) mice the odontoblast in the mandibular incisors showed weekly expression of DSPP 5. In the wild mice, the odontoblasts of mandibular incisors were not expression of BSP, but in the NFI-C (-/ -) mice the odontoblast of mandibular incisors showed strong expression of BSP These results suggest that odontoblast in the NFI-C (-/-) mice changes the phenotype into osteoblast. |
| Key Words:
NFI-C, Odontoblast differentiation, DSPP, BSP |
|
PDF Links
Download Citation
