THE EFFECT OF XYLITOL ON THE EXPRESSION OF GTF GENE

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Journal of the Korean Academy of Pediatric Dentistry 2004;31(2):304-313.
Published online May 31, 2004.

THE EFFECT OF XYLITOL ON THE EXPRESSION OF GTF GENE

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²

gtf 유전자 발현에 대한 xylitol의 영향

염정현¹, 정진², 정태성¹, 김신¹

¹부산대학교 치과대학 소아치과학교실
²부산대학교 치과대학 구강미생물학교실

Correspondence: ,

Abstract

Xylitol, a five-carbon natural sugar alcohol, is widely used non-cariogenic sugar substitute. In present study, the effects of xylitol on the expression of mRNA for glucosyltransferase which synthesizes glucan from sucrose were detected by Fluorescent in situ hybridization (FISH) and flow cytometry. FITC fluorescences for mRNA of gtfB, gtfC and gtfD were decreased further with increasing concentration of xylitol from 1% to 10% when detected by FISH. Flow cytometric analysis also showed that the expression of gtfB, gtfC and gtfD was increased by the addition of sucrose and decreased by the addition of xylitol to BHI broth containing 1% sucrose. In conclusion, the expression of gtfB, gtfC and gtfD mRNA was decreased by the addition of xylitol.

Key Words: Xylitol, Streptococcus mutans, gtf gene, mRNA, FISH, Flow cytometry