| Enhancement of Erythrosine Photodynamic Therapy against Streptococcus mutans by Chlorhexidine |
|
Jongcheol Park1, Howon Park1, Siyoung Lee2 |
1Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University
2Department of Microbiology and Immunology, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University |
| Streptococcus mutans에 대한 클로르헥시딘과 Erythrosine 광역동 치료의 상승효과 |
|
박종철1, 박호원1, 이시영2 |
1강릉원주대학교 치과대학 1소아치과학교실
2강릉원주대학교 치과대학 미생물학 및 면역학교실 및 구강과학연구소 |
Correspondence:
Howon Park, Tel: +82-33-640-2464, Fax: +82-33-640-3113, Email: pedo@gwnu.ac.kr
|
|
Received: 12 April 2013 • Accepted: 7 September 2013 |
|
|
| Abstract |
|
The purpose of this study was to investigate the synergistic effects of erythrosine sensitized with a conventional halogen curing unit and sub-minimal bactericidal concentration(sub-MBC) of chorhexidine on bacterial viability of Streptococcus mutans in planktonic state.
Sub-minimal bactericidal concentration of chlorhexidine was added into wells containing bacteria and erythrosine.
The range of concentrations tested for chorhexidine was from 0.0000001% to 0.001%. The irradiation of the bacterial suspensions was performed for 15 sec with a conventional halogen curing unit light. In another set of experiment, the effects of 0.001% chlorhexidine were observed by adding chlorhexidine into wells containing the sub-minimal bactericidal concentration of erythrosine.
At the concetration of 0.001% chlorhexidine, there were no antibacterial effects in the absence of erythrosine PDT(p < 0.05). At the concentraton of 1 μM erythrosine, there was no photodynamic therapy effect in the absence of chlorhexidine(p < 0.05). But in the presence of sub-minimal bactericidal concentration of erythrosine with light exposure, the addition of 0.001% chlorhexidine increased the bactericidal rate(p < 0.05).
A combination of erythrosine PDT with sub-MBC chlorhexidine resulted in a significant reduction in bacterial
counts when compared to the case with the absence of chlorhexidine. |
| Key Words:
Photodynamic therapy, Erythrosine, Halogen, Streptococcus mutans, Chlorhexidine |
|
PDF Links
Download Citation
