THE INHIBITORY EFFECT OF STREPTOCOCCUS SALIVARIUS 119 ON THE FORMATION OF ARTIFICIAL PLAQUE

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Journal of the Korean Academy of Pediatric Dentistry 2000;27(1):15-23.
Published online February 29, 2000.

THE INHIBITORY EFFECT OF STREPTOCOCCUS SALIVARIUS 119 ON THE FORMATION OF ARTIFICIAL PLAQUE

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Streptococcus salivarius 119의 인공치태 억제효과에 대한 연구

이민하¹, 양규호¹, 오종석²

¹전남대학교 치과대학 소아치과학교실
²전남대학교 의과대학 미생물학교실

Abstract

Streptococcus salivarius is a normal inhabitant in the human oral cavity. Streptococcus salivarius 119 in this study was isolated from the oral cavity of child and identified, and its action mechanism on the formation of denal plaque by Streptococcus matans was studied. 1. The optical density of absorbance at 550 nm was 0.327 in the culture of Streptococcus mutans in disposable cuvette, whereas being 0.119 in the combined culture of Streptococcus mutans and Streptococcus salivarius 119. 2. The mean weight of produced artificial plaque on the wires in the beaker was 84.7mg in culture of Streptococcus mutans only, whereas being reduced to 12.3mg in the combined culture of Streptococcus mutans and Streptococcus salivarius 119. 3. When Streptococcus mutans was cultured in the media containing culture supernatant of Streptococcus salivarius 119 in BHI broth, the mean weight of produced artificial plaque was 100.5mg on the wires, whereas being reduced to 20.4mg in the media containing culture supernatant of Streptococcus salivarius 119 in BHIS broth. 4. The viable cells of Streptococcus oralis and Streptococcus salivarius 119 were 4.8×10⁷and 7.5×10⁸ per ml respectively after each culture, wheras being 4.2×10⁷ and 5.8×10⁷ per ml in the combined culture of Streptococcus oralis and Streptococcus salivarius 119. 5. The polymer produced by Streptococcus salivarius 119 was glucan on the thin layer chromatography. 6. The glucan produced by Streptococcus salivarius 119 was water-soluble glucan containing 1→6 linkages as the main linkage on the thin layer chromatography. These results suggested that isolated Streptococcus salivarius 119 inhibited the formation of artificial plaque by the production of water-soluble glucan.

Key Words: Plaque, Glucan, Dental caries