EVALUATION OF THE GENOTOXICITY OF FERRIC SULFATE BY COMET ASSAY

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Journal of the Korean Academy of Pediatric Dentistry 2000;27(1):77-84.
Published online February 28, 2000.

EVALUATION OF THE GENOTOXICITY OF FERRIC SULFATE BY COMET ASSAY

omet assay를 이용한 Ferric Sulfate의 유전자 독성에 대한 연구

강호승, 김신, 정태성, 박혜련

부산대학교 치과대학 소아치과학교실

Abstract

Although ferric sulfate has been proposed as an alternative to formocresol in pulpotomy treatment in primary teeth, it has been given little concern regarding its cytotoxicity and mutagenicity. In the present study, we assessed the in vitro genotoxic effect of a ferric sulfate on human gingival fibroblast cell line (HGF-1). DNA damage was evaluated using comet assay (single cell alkaline gel electrophoresis) and obtained the results as follows: 1. A dose-response relationship was found between ferric sulfate concentrations (0 to 5mM) and DNA damages. 2. Above the concentration of 0.1mM, DNA damage was significantly increased than those of the control (p<0.05). 2. At the fixed concentration of 0.05mM, no significant difference was found between exposure time and DNA damage. These findings suggest that ferric sulfate as a pulpotomy agent can induce DNA damage in human gingival fibroblasts.

Key Words: Pulpotomy, Ferric sulfate, Genotoxicity, Comet assay