Journal of the Korean Academy of Pediatric Dentistry 2011;38(2):170-178.
Published online May 31, 2011.
PERIODONTOPATHIC BACTERIA AND ANTIBIOTIC RESISTANCE GENES OF ORAL BIOFILMS IN CHILDREN
1전남대학교 치의학전문대학원 소아치과학교실 및 치의학연구소
2전남대학교 치의학전문대학원 치의학교육학교실 및 치주학교실
3전남대학교 치의학전문대학원 교정학교실
4전남대학교 치의학전문대학원 치의학교육학교실
5전남대학교 의과대학 미생물학교실 
어린이 치면세균막에서 치주질환원인균과 항생제 내성유전자의 출현율
김선미1, 최남기1, 조성훈1, 이석우2, 임회정3, 임회순4, 강미선5, 오종석5
1전남대학교 치의학전문대학원 소아치과학교실 및 치의학연구소
2전남대학교 치의학전문대학원 치의학교육학교실 및 치주학교실
3전남대학교 치의학전문대학원 교정학교실
4전남대학교 치의학전문대학원 치의학교육학교실
5전남대학교 의과대학 미생물학교실
Correspondence:  , 
Received: 13 April 2011   • Accepted: 10 May 2011
Abstract
The purpose of this study was to assess the prevalence of periodontopathic bacteria and resistance determinants from oral biofilm of children. Subgingival dental plaque was isolated from 87 healthy children, and PCR was performed to determine the presence of 5 periodontal pathogens including P. gingivalis, T. forsythia, T. denticola, F. nucleatum, A. actinomycetemcomitans, and nine resistance genes including tet(Q), tet(M), ermF, aacA-aphD, cfxA, blaSHV, blaTEM, vanA, mecA. 1. The prevalence of F. nucleatum, T. forsythia. and P. gingivalis was 95.4%, 55.2%, and 40.2%, respectively. In addition. the prevalence of A. actinomycetemc omitans was 5.7%, while T. denticola was 3.4%. 2. In analysis of antibiotic resistance determinants. cfxA, blaTEM and tet(M) were detected in all the samples tested. It was also found that the prevalence of tet(Q) showing tetracycline resistance. blaSHV associated with resistance to β-lactams, ermF exhibiting erythromycin resistance, and, vanA resulting vancomycin resistance was 88.5%, 29.9% 87.4%, and 48.5%, respectively. The aacA-aphD gene showing resistance to aminoglycosides and mecA gene harboring methicillin resistance exhibited the lowest prevalence with 9.2%. 3. In a correlation analysis between periodontopathic pathogens and antibiotic resistance determinants, it was found that there was a significant correlation between T. forsythia and blaSHV. Also, P. gingivalis and vanA showed a correlation. Finally, tet(Q) and ermF showed a significant correlation (phi: 0.514) while mecA and vanA also showed a correlation(phi: 0.25).
Key Words: Periodontopathic bacteria, Antibiotic resistance, Antibiotic resistance determinants, Oral biofilm, Polymerase chain reaction
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