Journal of the Korean Academy of Pediatric Dentistry 2001;28(2):207-218.
Published online May 31, 2001.
A effect of calcium hydroxide endodontic materials on the differentiation and the activation of osteoclast
Eun-Young Jang1, Hoon Kwon1, Chang-Hee Lee2, Chang-Seop Lee1, Sang-Ho Lee1
1Department of Pediatric Dentistry, College of Dentistry, Chosun University
2Department of Microbiology, College of Dentistry, Chosun University 
수산화칼슘계 근관 충전제가 파골 세포의 분화 및 활성에 미치는 영향
장은영1, 권훈1, 이장희2, 이창섭1, 이상호1
1조선대학교 치과대학 소아치과학교실
2조선대학교 치과대학 미생물학교실
The purpose of this study was to investigate the direct inhibitory effect of calcium hydroxide materials on differentiation and activation of osteoclast. we used the osteoclast progenitor cells isolated from bone marrow cell of chick embryo tibia and four experimental materials [Ca(OH)2 powder, Metapaste®, Vitapex®, Pulpdent®] diluted at 0.1, 0.01, 0.05μg/ml. There were measured both the number of differentiated osteoclast and the area of resorption lacunae. Also, we conducted MTT assay on U2OS osteoblast to examine of cytotoxic effect and obtained following result. 1. Considering the result of the inhibitory effects upon osteoclast differentiation, There were shown a significant difference increased in the following order: Metapaste®, Ca(OH)2 powder, Vitapex®. But no significant difference was found in pulpdent group that the number of differentiated osteoclast was increased at all concentrations(p<0.05). 2. Among the three experimental groups, that is, Ca(OH)2 powder, Metapaste®, Vitapex® at $0.1μg/ml dilution that were statistically significant in reduction of the number of differentiated osteoclast. Vitapex group showed significant cytotoxic effect compared to control and another two groups exhibited no significant difference. Also, 0.2% DMSO group was shown statistically siginificant cytotoxicity (p<0.05). 3. Examining pattern and measured area of resorption lacunae in the control and the three experimental groups ,that is, Ca(OH)2 powder, Metapaste®, Vitapex® at 0.1μg/ml dilution, except Ca(OH)2 powder group, statistically significant differences were found between experimental groups and control group. Also, DMSO group showed statistically significant decrease (p<0.05). From these results, we think that calcium hydroxide is responsible for suppression of hard tissue resorption by a direct inhibition of dfferentiation and activation of osteoclast.
Key Words: Calcium hydroxide, Osteoclast, Differentiation, Activation, Inhibitory effect.

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