Journal of the Korean Academy of Pediatric Dentistry 2007;34(3):438-446.
Published online August 31, 2007.
The Effect of EGF, T3 and HB-EGF on Human Periodontal Fibroblasts
Eun-Kyoung Hong1, Jeong-Heon Cha1, Yun-Tae Kim2, Byung-Jai Choi1, Seong-Oh Kim1
1Department of Pediatric Dentistry, Department of Oral Biology, College of Dentistry and Oral Science Research Center
2Yonsei University, Daewoong Life Science Institute 
EGF, T3, HB-EGF 가 치주인대섬유모세포에 미치는 영향
홍은경1, 차정헌1, 김연태2, 최병재1, 김성오1
1연세대학교 치과대학 소아치과학교실, 구강생물학교실
2연세대학교 구강과학연구소, 대웅생명과학연구소
Correspondence:  Seong-Oh Kim,  Tel: 02-2228-3171, Fax: 02-392-7420, Email:
Viable cells of periodontal ligament would be an important factor for the successful replantation of an avulsed tooth. Therefore, it is critical to choose the storage medium for the preservation of traumatically avulsed teeth. Growth factors and hormones could be considered for the therapeutic application of the maintenance of viable periodontal ligament fibroblasts (PDLFs). Epidermal growth factor (EGF) has been suggested as an important player for the regeneration and wound healing process on other tissues. Therefore, EGF was evaluated for the therapeutic application on avulsed teeth. In addition, the synergic effect of EGF with tri-iodothyronine (T3) and heparin-binding epidermal growth factor-like growth factor (HB-EGF). The cell proliferation of PDLFs was determined by MTT assay and increased dose-dependently up to 10 ng/ml in the presence of EGF. Maximum cellular growth was shown at the concentration of 10 ng/ml EGF. Also, EGF promoted the wound healing of PDLFs examined by in vitro wound healing assay. Combined effects of EGF with T3 or HB-EGF on the proliferation of PDLFs were also studied. Interestingly, EGF showed the synergic effect on the proliferation of PDLFs with T3 and HB-EGF. To find out the mechanism of the synergic effect of EGF and T3, the effect of T3 on the expression of endogenous EGF receptor was determined by RT-PCR. The result was that T3 enhanced the expression of EGF receptor in PDLFs. It suggested that EGF might be a good choice for a therapeutic application, which can be used as combination with T3 and HB-EGF.
Key Words: Epidermal growth factor, Tri-iodothyronine, Heparin-binding epidermal growth factor-like growth factor, Periodontal ligament fibroblasts, Tooth avulsion
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