Journal of the Korean Academy of Pediatric Dentistry 2007;34(2):247-254.
Published online May 31, 2007.
PREVALENCE OF STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SOBRINUS IN CHILDREN WITH MIXED DENTITION
1경희대학교 치과대학 소아치과학교실 및 구강생물학연구소
2가이드치과병원 
혼합치열기 어린이에서 Streptococcus mutans와 Streptococcus sobrinus의 분포도 조사
이명성2, 최성철1, 박재홍1
1경희대학교 치과대학 소아치과학교실 및 구강생물학연구소
2가이드치과병원
Correspondence:  , 
Abstract
Mutans streptococci have been reported to be implicated in dental caries. Of these streptococcal species, Streptcoccus mutans and Streptococcus sobrinus are most commonly found in human dental caries. Prevalence of these bacterial species in dental caries is found to be varied in different races and countries. Therefore, importance of these bacteria in dental caries remains to be determined The present study was performed to find out correlation S. mutans and S. sobrinus with dental caries in 125 Korean children with mixed dentition between 6 to 11 years of age. They were classified as group A(6-8 years) and group B(9-11 years) by age. For the study, stimulated saliva samples were collected from each subject. The vials containing saliva specimens were serially diluted (1:10) in saline and plated in duplicate on tryptone-yeast extract-cysteine with sucrose and bacitracin (TYCSB) for S. mutans and S. sobrinus. After genomic DNA was extracted from the samples, polymerase chain reaction (PCR) amplification was performed for identification using universal primers and specific primers to S. mutans and S. sobrinus. Data of microbial variables were compared to caries status of the subjects. According to this study, the result were as follows : 1. S. mutans versus S. sobrinus were moderate positive linear correlated in both group A(r=0.70) and group B(r=0.50). 2. Between S. mutans and dental caries there were weak positive linear correlation in both group A(r= 0.25) and group B(r=0.34). 3. S. sobrinus versus dental caries were not correlated in group A but slightly correlated in group B(r=0.21). 4. Between S. mutans and age, there were not correlation in both group. 5. S. sobrinus versus age were weak correlated in group A(r=0.32) but not correlated in group B.
Key Words: Mixed dentition, S. mutans, S. sobrinus, Dental caries; Stimulated saliva


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