THE EFFECTS OF HYPERBARIC OXYGEN THERAPY ON PERIODONTAL WOUND HEALING OF REPLANTED RAT TOOTH

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Journal of the Korean Academy of Pediatric Dentistry 1997;24(1):41-57.
Published online February 28, 1997.

THE EFFECTS OF HYPERBARIC OXYGEN THERAPY ON PERIODONTAL WOUND HEALING OF REPLANTED RAT TOOTH

고압산소요법이 재식치아 치주조직치유에 미치는 영향

정일영, 김광철, 이긍호

경희대학교 치과대학 소아치과학 교실

Abstract

he present study investigated the effects of hyperbaric oxygen therapy on periodontal wound healing of replanted rat tooth. 80 rats (Sprague-Dawley strain) weighting 130±5gm were selected and divided into experimental and control group, each group consisting of 40 rats. Rats were administered 0.4% β-aminoproprionitrile for 5 days to achieve gentle tooth extraction. The maxillary first molars were extracted under anesthesia with pentobarbital, washed in sterile distilled water, treated with bacterial collagenase to remove collagen fibers on the root surfaces. After washing in water overnight, the mesial root surface were demineralized by application of citric acid, washed, dried and stored at 4℃. Immediately after tooth extraction and bleeding control, the treated molars extracted previously from other rats were replanted. The experimental group was exposed to hyperbaric oxygen at 2.5 atm. for 2 hrs. a day during experimental period. Eight animals of each group were sacrificed 1, 3, 6, 8, 10 days after reimplantation of teeth by intracardiac perfusion with 4% paraformaldehyde. The replanted molars and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin. Sections were stained with azan, toluidine blue and hematoxylin. Some other sections were stained by means of immunostaining achieved by the avidinbiotin complex method. The results as follows; 1. Experimental group showed fast healing of gingival epithelium. 2. Macrophage and newly formed blood vessels appeared early in the gingival connective tissue of experimental group. 3. Experimental group showed fast, abundant fibroblast proliferation and regularity of collagen fiber. 4. In both group, collagen was distributed along the collagen fiber. The distribution was strong and regular in the experimental group. 5. In the regenerated periodontal ligament of experimental group, fibers showed regular arrangement and invaded root surface fast.